In vitro chemotherapeutic and antiangiogenic properties of cardenolides from Acokanthera oblongifolia (Hochst.) Codd
Autor: | Amal Z. Hassan, Maha M. Soltan, Atef G. Hanna, Howaida I. Abd-Alla |
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Rok vydání: | 2021 |
Předmět: |
0303 health sciences
Programmed cell death medicine.diagnostic_test 010405 organic chemistry Cancer medicine.disease 01 natural sciences General Biochemistry Genetics and Molecular Biology In vitro 0104 chemical sciences Flow cytometry Vascular endothelial growth factor 03 medical and health sciences chemistry.chemical_compound chemistry Apoptosis Cancer cell Cancer research medicine Clonogenic assay 030304 developmental biology |
Zdroj: | Zeitschrift für Naturforschung C. 76:337-346 |
ISSN: | 1865-7125 0939-5075 |
DOI: | 10.1515/znc-2020-0302 |
Popis: | Acovenoside A (Acov-A) and acobioside A (Acob-A) were isolated from Acokanthera oblongifolia. Their anticancer properties were explored regarding, antiproliferative and antiangiogenic activities. The study included screening phase against six cancer cell lines followed by mechanistic investigation against HepG2 cancer cell line. The sulforhodamine-B (SRB) was used to determine their growth inhibitory power. In the other hand, flow cytometry techniques were recorded the cell death type and cell cycle analysis. The clonogenic (colony formation) and wound healing assays, enzyme-linked immunosorbent assay (ELISA) and molecular docking, were performed to evaluate the antiangiogenesis capability. Both compounds were strongly, inhibited four cancer cell lines at GI50 less than 100 nM. The in vitro mechanistic investigation against HepG2 resulted in cell accumulations at G2M phase and induction of apoptosis upon treating cells separately, with 400 nM Acov-A and 200 nM Acob-A. Interestingly, the same concentrations were able to activate caspase-3 by 7.2 and 4.8-fold, respectively. Suppressing the clonogenic capacity of HepG2 cells (20 and 40 nM) and inhibiting the migration of the colon Caco-2 cancer cells were provoke the results of vascular endothelial growth factor receptor2 (VEGFR2) kinase enzyme inactivation. The docked study was highly supportive, to the antiangiogenic approach of both cardenolides. The isolated cardenolides could orchestrate pivotal events in fighting cancer. |
Databáze: | OpenAIRE |
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