| Popis: |
We have developed PotatoMASH (Potato Multi-Allele Scanning Haplotags), a novel low cost, genome-scanning marker platform. We designed a panel of 339 multi-allelic regions placed at 1 Mb intervals throughout the euchromatic portion of the genome. These regions were assayed using a multiplex amplicon sequencing approach, which allows to genotype hundreds of plants at a cost of €5/sample. This protocol describes the library construction part of the method. PotatoMASH libraries are made to be sequenced in Illumina sequencing platforms. |
