The alkaline denaturation of arachin
| Autor: | W. E. F. Naismith, R. K. Williams |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Journal of Applied Chemistry. 6:421-427 |
| ISSN: | 1934-998X 0021-8871 |
| Popis: | The denaturation of arachin at pH 11 and at ionic strengths I = 1.0, 0.2 and 0.05 has been studied by means of the ultracentrifuge. Change of the arachin component to slowly sedimenting material occurred by way of an intermediate component of sedimentation constant similar to that of the arachin dissociation component. The rate cf disappearance of the arachin component is greater the lower the ionic strength whereas the formation of the intermediate component is favoured at high ionic strengths (e.g., 1.0). A fairly pure sample of this intermediate component has been prepared. The half life (t1/2) of the reaction at I = 0.2 was not found to vary significantly on increasing the protein concentration thirteenfold. The logarithmic plot of protein concentration against time, however, failed to give the straight line expected of a reaction which is first-order with respect to protein concentration. Possible causes for this seeming discrepancy are discussed. |
| Databáze: | OpenAIRE |
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