Analytical Assessment of a Home Made Capillary Electrophoresis Equipment with Linear Charge Coupled Device for Visible Light Absorption Detection in the Determination of Food Dyes
| Autor: | Renê S. Pinto, Emanuel Carrilho, Nilson Antonio Assunção, Karina Fraige |
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| Rok vydání: | 2009 |
| Předmět: |
Detection limit
Chromatography medicine.diagnostic_test Chemistry Clinical Biochemistry Analytical chemistry Pharmaceutical Science Biochemistry Analytical Chemistry chemistry.chemical_compound Electrophoresis Capillary electrophoresis Brilliant Blue FCF Fast Green FCF Spectrophotometry Sunset Yellow FCF medicine Visible spectrum |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 32:1862-1878 |
| ISSN: | 1520-572X 1082-6076 |
| DOI: | 10.1080/10826070903091571 |
| Popis: | The performance of modular home made capillary electrophoresis equipment with spectrophotometric detection, at a visible region by means of a miniaturized linear charge coupled device, was evaluated for the determination of four food dyes. This system presents a simple but efficient home made cell detection scheme. A computer program that converts the spectral data after each run into the electropherograms was developed to evaluate the analytical parameters. The dyes selected for analytical evaluation of the system were Brilliant Blue FCF, Fast Green FCF, Sunset Yellow FCF, and Amaranth. Separation was carried out in a 29 cm length and 75 µm I.D fused silica capillary, using 10 mmol L−1 borate buffer at pH 9, with separation voltage of 7.5 kV. The detection limits for the dyes were between 0.3 and 1.5 mg L−1 and the method presented adequate linearity over the ranges studied, with correlation coefficients greater than 0.99. The method was applied for determination and quantification of these dyes... |
| Databáze: | OpenAIRE |
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