Precipitation and Microflotation of Egg Albumin and Hemoglobin in the Presence of Dioctadecyldimethylammonmin Chloride
| Autor: | Haruo Nakayama, Kazuo Itoh, K\={o}z\={o} Shinoda, Egon Matijevic |
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| Rok vydání: | 1980 |
| Předmět: | |
| Zdroj: | Bulletin of the Chemical Society of Japan. 53:2493-2498 |
| ISSN: | 1348-0634 0009-2673 |
| DOI: | 10.1246/bcsj.53.2493 |
| Popis: | Precipitation and microflotation of egg albumin and of hemoglobin was studied as a function of pH in the presence of dioctadecyldimethylammonium chloride (DOAC). The “DOAC concentration-pH” stability domains for both protein solutions (0.05 wt%) exhibited similar boundaries. Efficient separation by precipitation (more than 80% after 24 h) occurs at pH>5.0 for albumin and at pH>6.3 for hemoglobin. The same amount of removal by microflotation required 5 min under otherwise similar conditions. The DOAC concentration for optimal separation of proteins (from 0.05 wt% solutions) is 0.02 wt% at pH 6–8 for albumin and 0.015 wt% at pH 6.5–8 for hemoglobin. It is noteworthy that both proteins can be precipitated and also foam separated even in strongly alkaline media, such as at pH 12, where the usual flocculents (e.g., aluminum salts) cannot be employed. This work points out the usefulness of infinitely aggregating surfactants as microflotation agents. |
| Databáze: | OpenAIRE |
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