| Popis: |
1. 1. Under laboratory conditions it was possible to receive w- and s-eggs from queens of Formica polyctena (Foerster) in such large numbers that their RNA patterns could be compared during the whole embryonic development. 2. 2. The RNA amount was higher in w-eggs than in s-eggs, but the concentration in egg volume was similar in both egg types. 3. 3. The electrophoretic pattern of the RNA demonstrated similar bands for w- and s-eggs at which the low-molecular compounds of the NF band (nucleotide fraction) were higher in s-eggs than in w-eggs. Qualitative differences of the low-molecular nucleic acid compounds were, however, not detected. 4. 4. During embryogenesis the RNA content increased in both egg types. The RNA pattern was characterized by an increase of the rRNA precursors and a drastic decrease of the low-molecular compounds in the NF band, a corresponding distinct reduction of the nucleotide fraction and, especially an increase of uric acid. 5. 5. The DNA amount increased about 30-fold during embryogenesis beginning at the blastoderm stage; it was similar in both egg types. This result suggests that the larvae hatched from w- and s-eggs have the same cell number. 6. 6. The RNA pattern of the late embryonic stages was highly similar to that of the ovaries of activated queens. 7. 7. No differences could be found in the metabolism of nucleic acid in w- and s-eggs which would permit an interpretation of the blastogenic predisposition on the biochemical level. |
