Autor: | Andrea Schneider, Maria de Fátima Carvalho-Jonas, Rainer Jonas, Roselene Merkle, Sandra Aparecida Furlan |
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Rok vydání: | 2000 |
Předmět: |
Vitamin
biology Chemistry food and beverages Bioengineering General Medicine biology.organism_classification Applied Microbiology and Biotechnology Corn steep liquor chemistry.chemical_compound Biochemistry Kluyveromyces marxianus β d galactosidase biology.protein Fermentation Food science Beta-galactosidase Aeration Lactose Biotechnology |
Zdroj: | Biotechnology Letters. 22:589-593 |
ISSN: | 0141-5492 |
Popis: | A lactose-free, low-cost culture medium for the production of β-d-galactosidase by Kluyveromyces marxianus was formulated. At high aeration rates (2.2 vvm) and concentrations of 100 g sugar cane molasses l−1 as carbon source and 100 g corn steep liquor l−1 as vitamin and nitrogen source an enzyme production of 708 U l−1 h was achieved. This was 20% higher than using a medium that contained lactose which is considered the primary inductor of β-d-galactosidase synthesis. |
Databáze: | OpenAIRE |
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