Autor: Andrea Schneider, Maria de Fátima Carvalho-Jonas, Rainer Jonas, Roselene Merkle, Sandra Aparecida Furlan
Rok vydání: 2000
Předmět:
Zdroj: Biotechnology Letters. 22:589-593
ISSN: 0141-5492
Popis: A lactose-free, low-cost culture medium for the production of β-d-galactosidase by Kluyveromyces marxianus was formulated. At high aeration rates (2.2 vvm) and concentrations of 100 g sugar cane molasses l−1 as carbon source and 100 g corn steep liquor l−1 as vitamin and nitrogen source an enzyme production of 708 U l−1 h was achieved. This was 20% higher than using a medium that contained lactose which is considered the primary inductor of β-d-galactosidase synthesis.
Databáze: OpenAIRE