Active mixing of immobilised enzymatic system in microfluidic chip
| Autor: | Kirill A. Lukyanenko, Elena N. Esimbekova, I. A. Denisov, A. S. Yakimov, Anatoly Evstrapov, Anton Bukatin, K. I. Belousov, P. I. Belobrov |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Biomedical Engineering Mixing (process engineering) Flavin mononucleotide Bioengineering 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Chip 01 natural sciences 0104 chemical sciences chemistry.chemical_compound Membrane Microfluidic chip Chemical engineering chemistry Active mixing Reagent General Materials Science 0210 nano-technology Dissolution |
| Zdroj: | Micro & Nano Letters. 12:377-381 |
| ISSN: | 1750-0443 |
| DOI: | 10.1049/mnl.2016.0646 |
| Popis: | Parameters for sample introduction, dried reagents dissolution and mixing with sample for bienzyme system NAD(H):FMN-oxidoreductase and luciferase immobilised in microfluidic chip were successfully determined. Numerical simulations of reaction chamber geometry, flavin mononucleotide (FMN) escape from starch gel and mixing options were conducted to achieve higher sensitivity of bioluminescent reaction. Results of numerical simulations were verified experimentally. The active mixer for dried reagents was made from an electro-mechanical speaker's membrane which was connected to the input of the chip. Such a mixer provided better efficiency than a passive mixing, and it is simple enough for use in point-of-care devices with any systems based on immobilised enzymes in chips. |
| Databáze: | OpenAIRE |
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