Optimization of the cultivation conditions and the basic molecular tools for roseoflavin producer Streptomyces davawensis
| Autor: | D.V. Fedorovych, R. Kordiaka, A.O. Tsyrulnyk, K.V. Dmytruk, A.A. Sybirny |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology Roseoflavin Chemistry Streptomyces cinnabarinus fungi 030106 microbiology Bacillus cereus General Medicine Bacillus subtilis biology.organism_classification 03 medical and health sciences Transformation (genetics) chemistry.chemical_compound Biochemistry bacteria Micrococcus luteus DNA Bacteria |
| Zdroj: | Mikrobiolohichnyi Zhurnal. 78:2-10 |
| ISSN: | 2616-9258 1028-0987 |
| DOI: | 10.15407/microbiolj78.04.002 |
| Popis: | Roseoflavin (RoF), and its metabolic precursor 8-dimethylaminoriboflavin (AF), produced by Gram-positive bacteria Streptomyces davawensis and Streptomyces cinnabarinus, reveal a strong antibiotic effect against Staphyloccus aureus, Bacillus subtilis, Bacillus cereus, Micrococcus luteus and other Gram-positive bacteria and could be promising for developing the new effective antibacterial drugs. In this work, we present the optimization of basic molecular tools for S. davawensis including total DNA preparation, PCR amplification of target gene, cell transformation. In addition, the compositions of cultivation media were studied and the optimal cultivation conditions for increased of RoF production have been developed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|