Cytophotometric study of DNA-fuchsin complex in cultured mesophyll protoplasts ofNicotiana tabacum andVicia faba

Autor:	A. V. Nosov, I. N. Smolenskaya, Anna L. Nosova
Rok vydání:	1983
Předmět:	food and beverages Plant Science Horticulture Cell cycle Biology Protoplast Molecular biology In vitro Mitotic cycle chemistry.chemical_compound chemistry Botany Schiff test DNA
Zdroj:	Biologia Plantarum. 25:173-179
ISSN:	1573-8264 0006-3134
DOI:	10.1007/bf02902098
Popis:	IsolatedN. tabacum andV. faba protoplasts were used as a model to examine the DNA-fuchsin changes during the dedifferentiationin vitro. Using the pattern recognition method for the evaluation of cytophotometric data obtained we have established: 1) the major fraction of cells of the original tobacco and bean leaf tissue is in G1-period of the cell cycle; 2) the significant decrease to 1C DNA-fuchsin content in nuclei in the first hours of cultivation and then a reconstitution of 2C pool followed by entering of these cells into S-period of mitotic cycle; 3) only 2% of tobacco and 4% of bean mesophyll cells contain 4C DNA.
Databáze:	OpenAIRE
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