| Autor: |
César Fernández-Sánchez, Olga Ordeig, Stefanie Demming, Pedro Martinez Ortiz, Xavier Muñoz-Berbel, Andreu Llobera, Stephanus Büttgenbach |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
2011 16th International Solid-State Sensors, Actuators and Microsystems Conference. |
| DOI: |
10.1109/transducers.2011.5969230 |
| Popis: |
An integrated lab on a chip that enables simultaneous optical and electrochemical analysis working in continuous flow mode is presented. Both transducers are integrated into a single detection cell and operated simultaneously with no evidences of cross-talk and in agreement with theoretical predictions by Levich equation (electrochemical) and by Lambert-Beer law (absorbance). Their performance is demonstrated by continuous detection of lactate via a double enzymatic reaction with lactate oxidase (LOX) and horseradish peroxidase (HRP). The consumption of the enzymatic reaction mediator (ferrocyanide, [Fe(CN) 6 ]4−) is recorded by amperometry, while the product (ferricyanide, [Fe(CN) 6 ]3−) is quantified by absorbance. The ability to quantify reagent and product simultaneously increases the robustness of the system by making it self-verifying, in addition to the inherent advantages of microfluidic devices. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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