Molecular Cloning, Segmental Distribution and Ontogenetic Regulation of Cationic Amino Acid Transporter 2 in Pigs

Autor:	Ping-Wen Xu, Yan Zhang, Shigeng Zou, Zhiyi Huang, Jianjun Zuo, Xiangyan Zhou, Dingyuan Feng, Ai-Nlin Zhi
Rok vydání:	2009
Předmět:	chemistry.chemical_classification Messenger RNA Kidney Ileum Biology Molecular biology Amino acid Jejunum medicine.anatomical_structure chemistry medicine Duodenum Animal Science and Zoology Amino acid transporter Peptide sequence Food Science
Zdroj:	Asian-Australasian Journal of Animal Sciences. 22:712-720
ISSN:	1976-5517 1011-2367
DOI:	10.5713/ajas.2009.80469
Popis:	The goal of this study was to elucidate the expression and segmental distribution of the glomerular cationic amino acid metabolism transporter-2 (CAT-2) and thus to improve our understanding of porcine cationic amino acid transporters and amino acid absorption. Porcine CAT-2 was cloned, sequenced and characterized. The predicted amino acid sequence of porcine CAT-2 shared 86.1% and 92.1% identity with human and mouse CAT-2A, respectively. The tissue distribution patterns and ontogenic changes of CAT-2 mRNAs were determined by real-time Q-PCR. The results showed that porcine CAT-2 was highly expressed in the heart and intestinal tract (duodenum, ileum and jejunum). In addition, the mRNA of CAT-2 was found in liver, lung, kidney, brain and muscle. Within the intestinal tract, CAT-2 mRNA was most abundant in the ileum and rarely expressed in the duodenum. In the duodenum, the levels of CAT-2 mRNA reached their peak on day 7 (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c8373f9690a579ca0a2975378e6cb715 https://doi.org/10.5713/ajas.2009.80469 Zobrazit plný text záznamu