Antiplasmodial Activity of the n-Hexane Extract from Pleurotus ostreatus (Jacq. ex. Fr) P. Kumm

Autor:	R.W.M. Krause, Heinrich C. Hoppe, Michelle Isaacs, Chiazor P Onyia, K. A. Abo, Olutayo M. Adedokun, Elizabeth C Chukwu, OE Afieroho, Xavier Siwe Noundou, Osamuyi H Festus
Rok vydání:	2019
Předmět:	Ergosterol Traditional medicine biology Chemistry Pharmaceutical Science biology.organism_classification Sterol Edible mushroom HeLa chemistry.chemical_compound Lactate dehydrogenase Molecular Medicine Pleurotus ostreatus Cytotoxicity IC50
Zdroj:	Turkish Journal of Pharmaceutical Sciences. 16:37-42
ISSN:	2148-6247 1304-530X
DOI:	10.4274/tjps.18894
Popis:	Objectives Several mushrooms species have been reported to be nematophagous and antiprotozoan. This study reported the antiplasmodial and cytotoxic properties of the n-hexane extract from the edible mushroom Pleurotus ostreatus and the isolation of a sterol from the extract. Materials and Methods Antiplasmodial and cytotoxicity assays were done in vitro using the plasmodium lactate dehydrogenase assay and human HeLa cervical cell lines, respectively. The structure of the isolated compound from the n-hexane extract was elucidated using spectroscopic techniques. Results The n-hexane extract (yield: 0.93% w/w) showed dose dependent antiplasmodial activity with the trend in parasite inhibition of: chloroquine (IC50=0.016 μg/mL) > n-hexane extract (IC50=25.18 μg/mL). It also showed mild cytotoxicity (IC50>100 μg/mL; selectivity index >4) compared to the reference drug emetine (IC50=0.013 μg/mL). The known sterol, ergostan-5,7,22-trien-3-ol, was isolated and characterized from the extract. Conclusion This study reporting for the first time the antiplasmodial activity of P. ostreatus revealed its nutraceutical potential in the management of malaria.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c830d01c3803341123ba84ff7e944db6 https://doi.org/10.4274/tjps.18894 Zobrazit plný text záznamu Plný text ve formátu PDF