A symmetrical luminol based azo derivative for trimodal ratiometric Hg2+ sensing and its application to bioimaging in living cells
| Autor: | Kannapiran Rajendra kumar, Simon Deepa |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
010405 organic chemistry
General Chemical Engineering General Physics and Astronomy Infrared spectroscopy General Chemistry 010402 general chemistry Photochemistry 01 natural sciences Fluorescence 0104 chemical sciences Luminol law.invention chemistry.chemical_compound chemistry law Absorption (chemistry) Hydrogen peroxide Isomerization Chemiluminescence Cysteine |
| Zdroj: | Journal of Photochemistry and Photobiology A: Chemistry. 364:773-786 |
| ISSN: | 1010-6030 |
| DOI: | 10.1016/j.jphotochem.2018.07.013 |
| Popis: | A C2 symmetric azo derivative of luminol (AzoLum) was used for the selective and specific sensing of Hg2+ using three different modes, 'cis-trans' isomerisation, fluorescence quenching and chemiluminescence enhancement. Hg2+ addition to AzoLum triggered the formation of 'cis' -AzoLum from the mixture of 'cis' and 'trans' isomers, alongside the fluorescence quenching. Ratiometric sensing of Hg2+ in the concentration range from 1 ppb to 1000 ppb was studied by following 'cis-trans' isomerisation and fluorescence quenching using absorption and fluorescence spectroscopic techniques respectively. On adding cysteine, 'Cis'-AzoLum- Hg2+ reverts back to a mixture of 'cis' and 'trans' isomers with the regained fluorescence. The nature of a complex formed between AzoLum and Hg2+ was studied using IR spectroscopy, XRD, TGA and theoretical DFT calculations. With the characteristic phthalhydrazide groups of luminol, AzoLum was also found to sense Hg2+ ratiometrically using chemiluminescence measurements in the presence of hydrogen peroxide. Furthermore, AzoLum was successfully used for bioimaging of Hg2+ in live cells. |
| Databáze: | OpenAIRE |
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