Novel immunoassay and rapid immunoaffinity chromatography method for the detection and selective extraction of naringin inCitrus aurantium
| Autor: | Yue Zhang, Yan Zhao, Feng Shenglan, Baoping Qu, Jinjun Cheng, Huihua Qu, Qingguo Wang, Shuchen Liu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Chromatography
medicine.diagnostic_test 010405 organic chemistry medicine.drug_class Chemistry 010401 analytical chemistry Extraction (chemistry) Filtration and Separation Monoclonal antibody 01 natural sciences 0104 chemical sciences Analytical Chemistry Sepharose chemistry.chemical_compound Affinity chromatography Antibodies monoclonal Immunoassay medicine Naringin |
| Zdroj: | Journal of Separation Science. 39:1389-1398 |
| ISSN: | 1615-9306 |
| DOI: | 10.1002/jssc.201501034 |
| Popis: | In this work, a novel monoclonal antibody specific for naringin was prepared and characterized. Subsequently, an indirect competitive enzyme-linked immunosorbent assay for naringin was developed, with an effective range from 4.8 to 156 ng/mL naringin. Next, an immunoaffinity column was obtained by coupling anti-naringin monoclonal antibodies to CNBr-activated Sepharose 4B and a rapid immunoaffinity chromatography assay for naringin was developed. The immunoaffinity column was used to separate naringin from Citrus aurantium. The results showed that 1 g of the dry Sepharose 4B can couple 10 mg of immunoglobulin G. And the immunoaffinity column can efficiently and specifically capture approximately 250 μg of naringin without cross reacting with its structurally similar compounds. Moreover, our results indicate that the application of immunoaffinity chromatography can simplify the pretreatment and the isolation process greatly compared to conventional methods, providing a potential method for extracting the target component from structurally similar compounds in natural products. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|