| Autor: | David S. Robinson, Naureen Afzal, C. Forster, Helen M. North, Claire Domoney, Andrzej R. Hornostaj, Rod Casey |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
musculoskeletal diseases
Genetics Messenger RNA Mutation endocrine system diseases integumentary system biology Mutant food and beverages Plant Science General Medicine biology.organism_classification medicine.disease_cause Pisum enzymes and coenzymes (carbohydrates) Sativum medicine Restriction fragment length polymorphism Agronomy and Crop Science Gene Regulator gene |
| Zdroj: | Plant Molecular Biology. 39:1209-1220 |
| ISSN: | 0167-4412 |
| Popis: | A mutant line of Pisum fulvum was identified that lacked seed lipoxygenase-2 (LOX-2). The mutant phenotype was introgressed into a standard Pisum sativum cv. Birte to provide near-isogenic lines with or without seed LOX-2. Genetic analyses showed the mutation to behave as a single, recessive Mendelian gene. Northern and dot-blot analyses showed a large reduction in LOX-2 mRNA from developing seeds of the LOX-2-null mutant. A restriction fragment length polymorphism associated with the 5′ end of the LOX-2 gene(s) co-segregated with the null phenotype, indicating that the reduction of LOX-2 mRNA was neither a consequence of deletion of the LOX genes nor a consequence of the action of a genetically distant regulatory gene. Analysis of the 5′-flanking sequences of LOX-2 genes from Birte and the near-isogenic LOX-2-null mutant revealed a number of insertions, deletions and substitutions within the promoter from the LOX-2-null mutant that could be responsible for the null phenotype. Incubation of crude seed LOX preparations from Birte and the LOX-2-null mutant showed that the latter generated relatively less 13-hydroperoxides and also produced relatively more hydroxy- and ketoacid compounds that have implications for the fresh-frozen pea industry. |
| Databáze: | OpenAIRE |
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