Somaclonal variation in sugarcane through tissue culture and evaluation for quantitative and quality traits

Autor: M. Krishnamurthi, S. Rajeswari, Annamalai Anandan, S. Thirugnanakumar
Rok vydání: 2009
Předmět:
Zdroj: Euphytica. 168
ISSN: 1573-5060
0014-2336
DOI: 10.1007/s10681-009-9889-4
Popis: Eight genotypes of Saccharum officinarum were crossed with Saccharum spontaneum and 14 genotypes of S. officinarum were crossed with Erianthus arundinaceus. A total of 39 hybrids were evolved. These 39 hybrids were raised in the field and used as donor clones for in vitro culture studies. Plantlets were regenerated from 1-month-old callus. The grown up plants were transplanted to well prepared field, to study the variations generated for the biometric as well as for biochemical characters. There were significant differences between the donor clones and their sub clones for all the character of interest. The somatic segregation was gradual and wider, showing a range of divergence from the mean towards the end of the scale. Fifty-one sub clones were selected with commercial potential which have 13% fibre, 200 cm stalk length, 10 cm internode length and pure obtainable cane sugar per cent of 10.
Databáze: OpenAIRE