A study of the effects of cultivation variety, collection time, and climate on the amount of oleuropein in olive leaves

Autor:	Alireza Ghiasvand, fatemeh Raeisi, Payman Hashemi, M. Alborzi, B. Delfan
Rok vydání:	2010
Předmět:	Detection limit chemistry.chemical_compound Horticulture Olive leaf Chromatography chemistry Oleuropein Cold season Collection Time Extraction (chemistry) General Chemistry Cultivar Hplc method
Zdroj:	Acta Chromatographica. 22:133-140
ISSN:	2083-5736 1233-2356
DOI:	10.1556/achrom.22.2010.1.10
Popis:	Summary Effects of cultivation variety, collection time, and climate on amounts of oleuropein (OE), a strong antioxidant, in olive leaves were studied. A modified ultrasound-assisted method was used for efficient and quantitative extraction of OE from olive leaf samples before their analysis by a rapid HPLC method. Under the optimized conditions, the extraction was performed in two successive steps with an RSD better than 2.95% (n = 5) and a detection limit of 0.02 μg OE. The amount of OE was determined in twelve samples of olive leaves from Mary Zeitoon, Koroneiki, and Sevillana varieties cultivated in three different regions of Iran after collection in two different seasons. The results confirmed that the amounts of OE varied in different olive varieties with mean values of 127, 115, and 144 μg g−1, respectively, although statistical tests indicated a significant interaction of collection time and climate with olive variety. In general, the OE concentration was significantly higher in the cold season an...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c7f96ecf4b3b7f3b6371418fe4c80b2d https://doi.org/10.1556/achrom.22.2010.1.10 Zobrazit plný text záznamu