Molecular Characterization of Carbapenem/Colistin-Resistant Acinetobacter baumannii Clinical Isolates from Egypt by Whole-Genome Sequencing
| Autor: | Doaa Gamal, Paul G. Higgins, Nevine Fam, Amani El-Kholy, May S. Soliman, Reham M Wasfy, Sara H. Mohamed |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pharmacology Whole genome sequencing clone (Java method) Carbapenem biology 030106 microbiology Broth microdilution biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Antimicrobial Genome Acinetobacter baumannii Microbiology 03 medical and health sciences 0302 clinical medicine Infectious Diseases polycyclic compounds medicine Colistin bacteria Pharmacology (medical) 030212 general & internal medicine medicine.drug |
| Zdroj: | Infection and Drug Resistance. 13:4487-4493 |
| ISSN: | 1178-6973 |
| DOI: | 10.2147/idr.s288865 |
| Popis: | Purpose The rise of carbapenem-resistant A. baumannii (CRAB) is considered a public health problem limiting the treatment options. Our current work studied the emergence and mechanisms of colistin-resistance among CRAB isolates in Egypt. Materials and methods Seventeen clinically recovered A. baumannii were identified and screened for their antimicrobial susceptibilities using VITEK-2 system. Colistin susceptibility was evaluated using broth microdilution, and characterization of carbapenem/colistin resistance determinants was performed using whole-genome sequencing (Illumina MiSeq). Results About 52.9% (9/17) were colistin-resistant. PCR results revealed that all isolates carried bla OXA-51-like genes, bla OXA-23-like was detected in 82.3% (14/17) and bla NDM in 23.5% (4/17). Two isolates harboured bla GES-35 and bla OXA-23. Furthermore, genome analysis of seven isolates revealed six belonged to international clone 2 (IC2) while the remaining isolate was a singleton (ST158), representing a clone circulating in Mediterranean/Middle Eastern countries. Conclusion The emergence and high incidence of colistin-resistance among CRAB clinical isolates in Egypt are alarming because it further limits therapy options and requires prudent antimicrobial stewardship and stringent infection control measures. Whole-genome sequence analyses suggest that the resistance to colistin was associated with multiple mutations in the pmrCAB genes. The high incidence of the high-risk lineage IC2 harbouring bla OXA-23-like as well as bla NDM is also of concern. |
| Databáze: | OpenAIRE |
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