Simultaneous determination of diclofenac sodium and paracetamol in a pharmaceutical preparation and in bulk drug powder by high-performance thin-layer chromatography

Autor: Vijay N. Gokarn, Ramesh T. Sane, Vidya Dighe, Sreedevi Pillai, Harsha N. Tambe, Shashikumar N. Menon
Rok vydání: 2006
Předmět:
Zdroj: Journal of Planar Chromatography – Modern TLC. 19:443-448
ISSN: 1789-0993
0933-4173
DOI: 10.1556/jpc.19.2006.6.7
Popis: A normal-phase high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous quantitative determination of diclofenac sodium and paracetamol in a pharmaceutical formulation and in bulk drug powder. The analysis was performed on silica gel 60F 254 HPTLC plates with toluene-ethyl acetate-methanol-formic acid, 5.0 + 4.0 + 1.0 + 0.01 ( v / v ), as mobile phase. Detection and quantitation were performed densitometrically at λ = 260 nm. Aceclofenac was used as the internal standard. Responses of diclofenac sodium standard and paracetamol standard were linear functions of concentration in the ranges 30–800 ng μL −1 and from 300–2000 ng μL −1 , respectively. The method was validated to determine its accuracy and precision. Accuracy was checked by conducting recovery studies; average recovery from the pharmaceutical preparation was 99.57% for diclofenac sodium and 100.51% for paracetamol. This HPTLC method developed for simultaneous quantitative determination of diclofenac sodium...
Databáze: OpenAIRE
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