Methodology for Identification of Cysteine-Reactive Covalent Inhibitors

Autor:	Stefan G. Kathman, Alexander V. Statsyuk
Rok vydání:	2019
Předmět:	chemistry.chemical_classification 0303 health sciences A protein 010402 general chemistry Mass spectrometry 01 natural sciences 0104 chemical sciences 03 medical and health sciences Enzyme chemistry Biochemistry Covalent bond Identification (biology) 030304 developmental biology Cysteine
Zdroj:	Methods in Molecular Biology ISBN: 9781493991860
DOI:	10.1007/978-1-4939-9187-7_15
Popis:	Covalent fragments are an emerging technology to discover covalent ligands in target-based or phenotypic screens. Here we describe screening of cysteine-reactive covalent fragments against a protein of interest using mass spectrometry or enzymatic methods.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c7d19cedecf859a35d48023346473099 https://doi.org/10.1007/978-1-4939-9187-7_15 Zobrazit plný text záznamu