| Autor: |
J. J. Lu, Ling Li, Ye-Qiang Liu, Yanqiu Li, Guanxin Shen, Lin Wang, Juan Tao, Tu Yt, Jiakuan Yang, F. Tian |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Clinical and Experimental Dermatology. 37:259-265 |
| ISSN: |
0307-6938 |
| Popis: |
Summary Background. Numerous molecular events have been associated with the development of malignant melanoma (MM). The cellular FLICE-like inhibitory protein (c-FLIP) was originally identified as an inhibitor of death-receptor signalling through competition with FADD-like interleukin-1β-converting enzyme (FLICE; also known as caspase-8) for recruitment to the FAS-associated death domain (FADD), and it has been suggested recently that c-FLIP is required for the survival and proliferation of various cell types. Aim. To investigate the relationship of c-FLIP expression with the clinicopathological features of MM. Methods. Immunohistochemical staining with anti-c-FLIP antibody was performed on tissue samples taken from 77 MMs and 20 naevi. The proliferative cells were visualized by staining with Ki-67 antibody. Annexin V–propidium iodide labelling, a marker for chromatin condensation, was performed to observe the rate of cell apoptosis by flow cytometry. Results. Expression of c-FLIP was increased in MM tissue compared with the matched pigmented naevi. The c-FLIP expression was significantly associated with the histological type and Clark level of MM, and correlated strongly with the Ki-67 labelling index. Downregulation of c-FLIP might increase apoptosis in MM cell lines. Conclusions. c-FLIP might play an important role in the obtaining of aggressive biological behaviours and be useful in predicting prognosis of patients with MM. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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