Histopathologic validation of 3′-deoxy-3′-18F-fluorothymidine PET for detecting tumor repopulation during fractionated radiotherapy of human FaDu squamous cell carcinoma in nude mice18F-FLT PET repopulation -->

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Autoři: Jia Yang, Guang Hui Bai, Jin Bo Yue, Yu-Hui Li, Jason D. Lee, Jing Liu, Alvin R. Cabrera, Jin Ming Yu, Xin Dong Sun
Zdroj: Radiotherapy and Oncology. 111:475-481
Informace o vydavateli: Elsevier BV, 2014.
Rok vydání: 2014
Témata: Pathology, medicine.medical_specialty, Fractionated radiotherapy, Chemistry, Hematology, 18f fluorothymidine, Oncology, In vivo, medicine, Immunohistochemistry, Radiology, Nuclear Medicine and imaging, Basal cell, Repopulation, Ex vivo, Accelerated repopulation
Popis: Background and purpose FaDu human squamous cell carcinoma (FaDu-hSCC) demonstrates accelerated tumor repopulation during fractionated irradiation with pathological validation (Ki-67 and BrdUrd makers) in a xenograft model system. However, these and other functional assays must be performed ex vivo and post hoc. We propose a novel, in vivo, real-time assay utilizing 18F-FLT PET. Material and methods Nude mice with FaDu-hSCC were irradiated with 12 or 18 fractions of 1.8 Gy ([Dm] = 3.0 Gy), either daily or every second day. 18F-FLT micro-PET scans were performed at different time points, FLT parameters (SUVmax, SUVmean, and T/NT) were measured. Tumor sections were stained for Ki-67 and BrdUrd, a labeling index (LI) was calculated. Imaging-pathology correlation was determined by comparing FLT parameters and immunohistochemical results. Results Measured SUVmax, SUVmean and T/NT decreased significantly after daily irradiation with 12 fractions in 12 days (P 0.05) and 18 fractions in 36 days (P > 0.05), suggesting accelerated repopulation. Similarly, Ki-67 and BrdUrd LIs demonstrated significant decreases with daily irradiation (P 0.05). 18F-FLT parameters correlated strongly with proliferation markers (r2: 0.679–0.879, P Conclusions 18F-FLT parameters were in good agreement with Ki-67 and BrdUrd Li. These results may support a potential role for 18F-FLT PET in real-time detection of tumor repopulation during fractionated radiotherapy.
ISSN: 0167-8140
Přístupová URL adresa: https://explore.openaire.eu/search/publication?articleId=doi_________::c7c8ff043116a80a1a250ec525418530
https://doi.org/10.1016/j.radonc.2014.04.002
Rights: CLOSED
Přírůstkové číslo: edsair.doi...........c7c8ff043116a80a1a250ec525418530
Autor: Jia Yang, Guang Hui Bai, Jin Bo Yue, Yu-Hui Li, Jason D. Lee, Jing Liu, Alvin R. Cabrera, Jin Ming Yu, Xin Dong Sun
Rok vydání: 2014
Předmět:
Zdroj: Radiotherapy and Oncology. 111:475-481
ISSN: 0167-8140
Popis: Background and purpose FaDu human squamous cell carcinoma (FaDu-hSCC) demonstrates accelerated tumor repopulation during fractionated irradiation with pathological validation (Ki-67 and BrdUrd makers) in a xenograft model system. However, these and other functional assays must be performed ex vivo and post hoc. We propose a novel, in vivo, real-time assay utilizing 18F-FLT PET. Material and methods Nude mice with FaDu-hSCC were irradiated with 12 or 18 fractions of 1.8 Gy ([Dm] = 3.0 Gy), either daily or every second day. 18F-FLT micro-PET scans were performed at different time points, FLT parameters (SUVmax, SUVmean, and T/NT) were measured. Tumor sections were stained for Ki-67 and BrdUrd, a labeling index (LI) was calculated. Imaging-pathology correlation was determined by comparing FLT parameters and immunohistochemical results. Results Measured SUVmax, SUVmean and T/NT decreased significantly after daily irradiation with 12 fractions in 12 days (P 0.05) and 18 fractions in 36 days (P > 0.05), suggesting accelerated repopulation. Similarly, Ki-67 and BrdUrd LIs demonstrated significant decreases with daily irradiation (P 0.05). 18F-FLT parameters correlated strongly with proliferation markers (r2: 0.679–0.879, P Conclusions 18F-FLT parameters were in good agreement with Ki-67 and BrdUrd Li. These results may support a potential role for 18F-FLT PET in real-time detection of tumor repopulation during fractionated radiotherapy.
Databáze: OpenAIRE
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