In vitro analysis of Mg scaffolds coated with polymer/hydrogel/ceramic composite layers
| Autor: | Lobat Tayebi, Mehdi Razavi, Gerwald A. Köhler, Daryoosh Vashaee, Mostafa Yazdimamaghani, Venkata Raveendra Pothineni, Jayakumar Rajadas, Senait Assefa |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Scaffold
Materials science food.ingredient 02 engineering and technology 010402 general chemistry 01 natural sciences Gelatin law.invention chemistry.chemical_compound food law Materials Chemistry Ceramic Viability assay chemistry.chemical_classification Surfaces and Interfaces General Chemistry Polymer 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Surfaces Coatings and Films chemistry visual_art Bioactive glass Polycaprolactone visual_art.visual_art_medium Surface modification 0210 nano-technology Biomedical engineering |
| Zdroj: | Surface and Coatings Technology. 301:126-132 |
| ISSN: | 0257-8972 |
| DOI: | 10.1016/j.surfcoat.2016.01.017 |
| Popis: | Magnesium (Mg), as a biodegradable metal, has recently been considered to be used in hard tissue engineering scaffold design. However, the fast release of hydrogen gas during exposure of Mg to corroding biofluids significantly limits the cytocompatibility of the scaffolds. To overcome this key drawback, in this study, the surfaces of Mg scaffolds are modified by polymer/hydrogel/ceramic layers consisting of polycaprolactone (PCL), gelatin (Gel) and bioactive glass (BaG). A detailed study has been performed on the in vitro mechanical properties of the Mg scaffold coated by PCL–BaG/Gel–BaG compared with the uncoated one. Our results show that the coated scaffold can keep its mechanical integrity three times longer than the uncoated one. To assess cytocompatibility, human osteoblast Saos-2 cells were cultured on the surface of the scaffolds. Cell attachment and growth were evaluated by scanning electron microscopy and cell viability assays, respectively. While no cell could attach on the uncoated scaffold, cell viability and growth are acceptable on the Mg scaffold/PCL–BaG/Gel–BaG. |
| Databáze: | OpenAIRE |
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