| Popis: |
Background: Heme oxygenase-1 (HO-1) is known to have anti-inflammatory, antiapoptotic and antioxidant effects in various diseases. In this study, we aimed to investigate whether HO-1 has a protective effect on isoprenaline (IP)-induced acute myocardial infarction (AMI) in rat.Materials and methods: Rats were received 200 mg/kg IP, alone or together with cobalt protoporphyrin (CoPP) and zinc protoporphyrin (ZnPP), an inducer and an inhibitor of HO-1, respectively. Four hours after the IP application, rats were killed. Blood and hearts were removed. Troponin-T (TnT) levels were assayed in serum as an indicator of AMI. Dien conjugate levels, protein carbonyl levels and antioxidant defense system are assayed in heart tissues by spectrophotometric methods. HO-1 protein levels were quantitated by western blot analysis in heart tissues. The results were compared with those obtained from control groups.Results: We confirmed AMI formation with higher serum TnT levels in IP-treated groups comparing to control groups. We found a significant elevation in HO-1 protein levels in IP plus CoPP-treated rats as compared to IP-treated rats, while serum TnT levels were observed to decrease at the same time. Protein carbonyl levels in heart tissues increased significantly in IP-treated groups compared with control groups. But, dien conjugate and antioxidant defense system remained unchanged.Conclusions: As a conclusion, we may suggest that HO-1 induction produces a protective effect against to myocardial damage occured by IP application in rat. |
