Nuclear isolation and sequencing for mouse hypothalamus v1
| Autor: | Zhengzheng Sophia Liang, Eric Vaughn, Dhananjay Bambah-Mukku, Catherine Dulac |
|---|---|
| Rok vydání: | 2021 |
| DOI: | 10.17504/protocols.io.bwr4pd8w |
| Popis: | This protocol is intended for isolation of nuclei from fresh brain tissue in preparation for single-nuclei sequencing library using Chromium Single Cell 3’ Reagent Kits v2 or v3 (10X Genomics). For each step, tissue and reagents were kept on ice or at 4C, to reduce unwanted gene expression due to procedures and maintain nuclear integrity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|