Vasopressin Messenger Ribonucleic Acid Regulation via the Protein Kinase A Pathway1
| Autor: | Charles H. Emerson, Joseph A. Majzoub, Zoya K. Arbiser, Yasumasa Iwasaki, Rodica L. Emanuel, Elena M. Velez |
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| Rok vydání: | 1998 |
| Předmět: | |
| Zdroj: | Endocrinology. 139:2831-2837 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.139.6.6043 |
| Popis: | Activation of vasopressin (VP) gene expression in vivo by osmotic stimuli results in an increase in both messenger RNA (mRNA) content and polyadenylate [poly(A)] tail length. VP gene transcription in vitro is stimulated by protein kinase A (PKA) activation. To examine the role of PKA in the regulation of VP mRNA poly(A) metabolism, constructs of the rat VP gene were permanently transfected into the mouse anterior pituitary cell line, AtT-20. Treatment with forskolin of cells expressing the intact VP gene resulted in increased VP gene transcription, an increase in the content of VP mRNA, and a shift toward VP mRNA species with longer poly(A) tails accompanied by the loss of VP mRNA species with shorter poly(A) tails. We uncoupled the PKA-stimulated appearance of long-tailed species from the disappearance of short-tailed species, suggesting that the size shift was caused by a coincident, but uncoupled net increase in VP mRNA species with elongated poly(A) tails and net loss of mRNA species with short poly(A... |
| Databáze: | OpenAIRE |
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