Hibiscus sabdariffaincreases hydroxocobalamin oral bioavailability and clinical efficacy in vitamin B12deficiency with neurological symptoms
Autor: | Kaies Rtibi, Mouna Loukili, Aslihan Özel, Seloua El Ouezzani, Nicolas Limas-Nzouzi, Z. Souirti, Imar Djibrine Soudy, Bruno Eto |
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Rok vydání: | 2016 |
Předmět: |
Pharmacology
business.industry Hibiscus sabdariffa Ileum Hydroxocobalamin Bioavailability 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Intestinal mucosa 030220 oncology & carcinogenesis Medicine Pharmacology (medical) Clinical efficacy Vitamin B12 business Adverse effect 030217 neurology & neurosurgery medicine.drug |
Zdroj: | Fundamental & Clinical Pharmacology. 30:568-576 |
ISSN: | 0767-3981 |
Popis: | The aim of the study was to evaluate the bioavailability and clinical benefits of oral new formulation (HB12 ) of hydroxocobalamin (Hdrx) with Hibiscus sabdariffa (HS). First, in an observational study, a cohort of 30 vitamin B12 -deficient patients (vit B12 < 200 pg/mL) with neurological symptoms received oral fixed dose of Hdrx containing 15 mg Hdrx daily for 10 days followed by 15 mg monthly. Clinical benefits were evaluated on haematological and biochemical parameters, and neurological improvement at days 10 and 90 compared to day 0. To understand the mechanism, intestinal mucosa from mice were mounted in vitro in Ussing chambers to measure Hdrx Fluxes. In the clinical study, serum vitamin B12 level increased from 55.1 ± 36.9 to 1330 ± 335.5 pg/mL at day 10 and 431.0 ± 24.27 pg/mL at day 90, without overt adverse effects. In mice ileum, (i) intestinal bioavailability of Hdrx increased in dose-dependent manner with HB12 . The apparent permeability of Hdrx was Papp = 34.9 ± 4.6 × 10-6 cm/s in the presence of 3 mg/mL (HB12 B) compared to the control Papp = 6.2 ± 0.7 × 10-6 cm/s. (ii) Total transepithelial electrical conductance (Gt ) increased in dose-dependent manner with HB12 , Gt = 161.5 ± 10.8 mS/cm² with HB12 B (Hdrx 1 mg + HS 3 mg) compared to the control Hdrx, Gt = 28.7 ± 4.0 mS/cm². In conclusion, the clinical study suggests that injections are not required when Hdrx is given orally. Intestinal bioavailability of Hdrx increased in vitro when it was used concomitantly with HS. |
Databáze: | OpenAIRE |
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