Triangular Phase Diagrams in the Phospholipid−Water−Alcohol System for the Preparation of Lipid Vesicles (Liposomes) Using the Coacervation Technique
| Autor: | Fumiyoshi Ishii, Katsuhiko Saegusa |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Coacervate
Chromatography Ethanol Sodium chemistry.chemical_element Surfaces and Interfaces Electrolyte Condensed Matter Physics Solvent chemistry.chemical_compound chemistry Sodium sulfate Electrochemistry lipids (amino acids peptides and proteins) General Materials Science Ammonium chloride Methanol Spectroscopy |
| Zdroj: | Langmuir. 18:5984-5988 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la0118412 |
| Popis: | The effect of alcohol and electrolytes on simple coacervation in the phospholipid−water−alcohol system is described in detail. The optimal coacervation conditions for formation of lipid vesicles in the phospholipid−water−alcohol system were examined by the triangular phase diagram. Three different alcohols, methanol, ethanol, and 1-propanol, were used as a lipid solvent. Water and various electrolyte solutions such as sodium chloride, ammonium chloride, magnesium chloride, calcium chloride, and sodium sulfate were used as a poor solvent for the lipids. When methanol and 1-propanol were used as lipid solvents, the entire phase resulted in a transparent, highly viscous gel phase. On the other hand, the profile of the coacervate precipitation of the phospholipid was observed when ethanol was used as a lipid solvent. Moreover, when 1-propanol was used as a lipid solvent, a large volume of water phase was required to induce the coacervation in the systems in comparison with methanol or ethanol. The coacervatio... |
| Databáze: | OpenAIRE |
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