A nanozyme-linked immunosorbent assay based on metal–organic frameworks (MOFs) for sensitive detection of aflatoxin B1
| Autor: | Yun-Hui Cheng, Yanqiu Chen, Mao-Long Chen, Ling-Li Long, Zhou Xu |
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| Rok vydání: | 2021 |
| Předmět: |
Detection limit
Aflatoxin Chromatography Chemistry Chromogenic 010401 analytical chemistry 04 agricultural and veterinary sciences General Medicine High sensitive 040401 food science 01 natural sciences 0104 chemical sciences Analytical Chemistry 0404 agricultural biotechnology Recovery rate Food Science |
| Zdroj: | Food Chemistry. 338:128039 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2020.128039 |
| Popis: | In order to avoid the occurrence of false positives and false negatives caused by conventional enzyme-linked immunosorbent assay (ELISA), we established a novel indirect competitive MOF-linked immunosorbent assay (MOFLISA) method for the high throughput and high sensitive detection of aflatoxin B1. This method replaces the natural enzyme with functional MOFs to catalyze a chromogenic system. As a result, the limit of detection (LOD) of the MOFLISA method was 0.009 ng·mL−1 with a linear working range from 0.01 to 20 ng·mL−1. The developed MOFLISA method for AFB1 has a 20-fold improved LOD value compared with the conventional ELISA. The recoveries and relative standard deviations (RSD) ranged from 86.41 to 99.74% and 2.38–9.04%, respectively. The results demonstrate that the recovery rate and accuracy of this detection method is better than that of conventional ELISA, reducing risks of false positive and false negative results. |
| Databáze: | OpenAIRE |
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