Low-molecular weight hyaluronidase from the venom ofBungarus caeruleus(Indian common krait) snake: Isolation and partial characterization
| Autor: | S.M.Veena, Sunil S. More, P. M. Sundaram, J. Bhavya, M S. Vineetha, Bhadrapura Lakkappa Dhananjaya |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Chromatography biology Chemistry Clinical Biochemistry Pharmaceutical Science Venom Context (language use) biology.organism_classification Biochemistry High-performance liquid chromatography Analytical Chemistry 03 medical and health sciences Bungarus 030104 developmental biology Enzyme Hyaluronidase Zymogen medicine Common krait medicine.drug |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 39:203-208 |
| ISSN: | 1520-572X 1082-6076 |
| Popis: | Snake venom hyaluronidases known as “spreading factor” are not extensively studied. Recently, it is argued that beyond its role as a spreading factor, venom hyaluronidase (HYL) deserves to be explored as a possible therapeutic target for inhibiting the systemic distribution of venom/toxins and also for minimizing local tissue destruction. In this context, in the present study, a low-molecular weight HYL has been isolated from Bungarus caeruleus (Indian krait) venom by single step chromatography on HPLC system. The apparent molecular weight determined by SDS-PAGE is 14 ± 2 kDa, as confirmed by zymogen study and LC–MS as well. The enzyme had optimal pH 6 and temperature 37°C. The Michaelis–Menten constant (Km) was found to be 8.48 µg/mL at 37°C. The activity of purified enzyme was completely inhibited by Ba2+ metal ion and N-acetyl imidazole group-specific agents. This work yielded a highly active HYL from B. caeruleus the first one to be isolated. Further studies on its pharmacological actions will... |
| Databáze: | OpenAIRE |
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