| Autor: |
Beata Klim, Pawel Laskowski, Dorota Lemancewicz, Janusz Dzięcioł, Karol Ostrowski, Katarzyna Kitlas, Maria M. Winnicka |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
American Journal of Pharmacology and Toxicology. 8:120-127 |
| ISSN: |
1557-4962 |
| DOI: |
10.3844/ajptsp.2013.120.127 |
| Popis: |
Mature cell release takes place through the hypothe tical barrier- the bone marrow-blood barrier. It co nsists of blood vessels and cells that surround them. Such ce lls are, among others, megakaryocytes. They are involved in the release of platelets or other cellular componen ts of blood. The amount and the development of megakaryocytes and thus the maturity of barrier dep ends on many factors. One of them is IL-6, which gene is located on the short arm of chromosome 7 (7p15-p21). The study was performed on wild-type mice (C57B4/6J) and on mice lacking the gene encoding IL-6 (IL-6 KO-C57B4/6J IL-6tm1 Kopf-/-). Bone marrow of all animals in both groups has been collected, fixed and staine d. Megakaryocytes were identified in each microscopic specimen using immunohistochemical reaction with the CD61 antibody. Then all specimens were subjected to histomorphometric analysis. Statistically significa nt differences in the total number and size of maga kariocytes was noted, which affects bone marrow-blood barrier functions. This could be used in the conventional t reatment of cancer, respiratory diseases and coagulation dis orders and in the treatment using nanoparticles. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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