Indole alkaloid production by transformed and non-transformed root cultures ofCatharanthus roseus

Autor:	B. Chí, L. M. Pacheco, J. Coello-Coello, M. L. Miranda-Ham, R. Ciau-Uitz, V. M. Loyola-Vargas
Rok vydání:	1994
Předmět:	Indole test Ajmalicine Rhizobiaceae Indole alkaloid Apocynaceae Alkaloid Plant Science Catharanthine Biology Catharanthus roseus biology.organism_classification chemistry.chemical_compound chemistry Botany heterocyclic compounds Biotechnology
Zdroj:	In Vitro Cellular & Developmental Biology - Plant. 30:84-88
ISSN:	1475-2689 1054-5476
DOI:	10.1007/bf02632126
Popis:	Ten transformed and two non-transformed root lines ofCatharanthus roseus were established. A systematic study of the growth kinetics and alkaloid content was performed over a culture cycle and showed significant differences between transformed and non-transformed cultures. Mean doubling times for transformed and normal root lines were 2.8 and 19.5 days, respectively. Alkaloid content in hairy roots was from two- to threefold higher than in the non-transformed tissues. The established transformed root lines produced a wide variety of indole alkaloids as can be observed from their complex thin layer chromatography patterns. A large quantity of serpentine was determined in two of the transformed root cultures. Alkaloid content, both quantitatively and qualitatively, has been stable in the hairy root cultures for more than 2 yr of subculturing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c76536d0aff10ff2ab42093dbdadccdb https://doi.org/10.1007/bf02632126 Zobrazit plný text záznamu Full text from SpringerLink