| Autor: |
Flávia Xavier, Fabio Daumas Nunes, David Livingstone Alves Figueiredo, Katiucia Paiva, Raquel Moyses, Maria Fernanda Rodrigues, Camila Rodini, Daniel Guariz Pinheiro, Patricia Severino, Adriana Madeira Álvares da Silva, Flavia Cristina Rodrigues-Lisoni, Pedro Guimaraes |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Journal of Oral Pathology & Medicine. 41:54-60 |
| ISSN: |
0904-2512 |
| DOI: |
10.1111/j.1600-0714.2011.01053.x |
| Popis: |
J Oral Pathol Med (2012) 41: 54–60 Background: Oral cancer overexpressed 1 (ORAOV1) was found as a candidate oncogene in the 11q13 chromosomal region, based on its amplification and overexpression in oral cancer cell lines. Because gene amplification often leads to increased levels of gene expression, we aimed to verify the relationship between ORAOV1 gene status and mRNA expression primarily in oral squamous cell carcinoma (OSCC) by quantitative assay, correlating with clinical and pathological characteristics in patients. Methods: Levels of ORAOV1 amplification and expression were evaluated by qPCR and RT–qPCR in OSCC cell lines and in tumor and non-tumoral surgical margins from 33 patients with OSCC. All subjects were smokers and habitual alcohol drinkers, mostly men above 40 years of age and with a single primary tumor. Results: ORAOV1 exhibited increased gene expression levels as well as higher copy number in three OSCC cell lines with 11q13 amplified chromosomal region when compared with the OSCC cell line without the amplification (one-way ANOVA, P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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