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Head Post and Cranial Window Outline Janelia Research Campus Svoboda Lab Developed by Karel Svoboda for Trachtenberg et al 2002 Improved by Anthony Holtmaat for Holtmaat et al 2006 and Holtmaat et al 2009 Improved by Daniel Huber for Huber et al 2012 Compiled by Bryan Maclennan (2013), Abridged version by Courtney Davis (2015) Compiled to protocols.io by JJ Kim (2019) Note: This outline only provides practical step-by-step advice. All surgeons should be familiar with and carefully adhere to the official animal protocol and current procedures posted on the Svoboda lab wiki. Trachtenberg, J. T., Chen, B. E., Knott, G. W., Feng, G., Sanes, J. R., Welker, E., & Svoboda, K. (2002). Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex. Nature, 420(6917), 788–794. https://doi.org/10.1038/nature01273 Holtmaat, A., Wilbrecht, L., Knott, G. W., Welker, E., & Svoboda, K. (2006). Experience-dependent and cell-type-specific spine growth in the neocortex. Nature, 441(7096), 979–983. https://doi.org/10.1038/nature04783 Holtmaat, A., Bonhoeffer, T., Chow, D. K., Chuckowree, J., De Paola, V., Hofer, S. B., Hübener, M., Keck, T., Knott, G., Lee, W. C. A., Mostany, R., Mrsic-Flogel, T. D., Nedivi, E., Portera-Cailliau, C., Svoboda, K., Trachtenberg, J. T., & Wilbrecht, L. (2009). Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window. Nature Protocols, 4(8), 1128–1144. https://doi.org/10.1038/nprot.2009.89 Huber, D., Gutnisky, D. A., Peron, S., O’Connor, D. H., Wiegert, J. S., Tian, L., Oertner, T. G., Looger, L. L., & Svoboda, K. (2012). Multiple dynamic representations in the motor cortex during sensorimotor learning. Nature, 484(7395), 473–478. https://doi.org/10.1038/nature11039 |
