Biphasic Effect of Calcium on Luteinizing Hormone-Stimulated Cyclic Adenosine 3′,5′-Monophosphate Production in Granulosa Cells of the Fowl (Gallus Domestics)1
| Autor: | Mohammad Jamaluddin, Miklós Molnár, Frank Hertelendy |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Calmodulin biology chemistry.chemical_element Cell Biology General Medicine Trifluoperazine Calcium Calcium Ionophores chemistry.chemical_compound Endocrinology Reproductive Medicine chemistry Internal medicine Ionomycin Second messenger system medicine biology.protein Extracellular Intracellular medicine.drug |
| Zdroj: | Biology of Reproduction. 46:698-704 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod46.4.698 |
| Popis: | Both cAMP and Ca2+ play important roles in the steroidogenic action of LH in hen granulosa cells. However, the interaction of these intracellular messengers is not fully understood. In the present study we used two calcium ionophores (ionomycin and A23187), as well as trifluoperazine (TFP), an inhibitor of calmodulin, to investigate LH- and forskolin-induced cAMP production in granulosa cells isolated from the largest (F1) preovulatory follicle of White Leghorn laying hens. Between 0.1 and 1.0 microM, both ionophores significantly potentiated cAMP responses to LH in the presence of 0.1 mM extracellular Ca2+. When calcium was omitted from the medium, ionophores had no effect. When either calcium was raised above 1 mM, or the concentration of ionophores was increased above 1 microM, LH-induced cAMP production was drastically inhibited. In the presence of 0.5-2.0 mM calcium, A23187 inhibited forskolin-promoted cAMP synthesis. TFP, while having no effect on basal cAMP, suppressed LH-induced responses and the potentiating effect of ionomycin. It is concluded that for full activation of the adenylate cyclase/cAMP system by LH, Ca-calmodulin is required at a site upstream from the catalytic component of the enzyme. However, high intracellular Ca2+ and/or other effects of ionophores (such as uncoupling of oxidative phosphorylation) inhibit LH-induced cAMP production. |
| Databáze: | OpenAIRE |
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