Characterization of a new family of proteins that interact with the C-terminal region of the chromatin-remodeling factor CHD-31

Autor:	Jörg Kobarg, Flávia C. Nery, Dario O. Passos, Taila Andrade Lemos
Rok vydání:	2002
Předmět:	HMG-box Biophysics Chromatin Remodeling Factor Cell Biology Biology Biochemistry Molecular biology Fusion protein humanities Chromatin remodeling Chromatin Structural Biology Cytoplasm TAF4 mental disorders Gene expression Genetics Molecular Biology
Zdroj:	FEBS Letters. 533:14-20
ISSN:	0014-5793
Popis:	The two human proteins Ki-1/57 and CGI-55 have highly similar amino acid sequences but their functions are unknown. We analyzed them by yeast two-hybrid screens and found that they interact with the C-terminal region of the human chromatin-remodeling factor CHD-3 (chromo-helicase-DNA-binding domain protein-3). The interaction of CGI-55 and CHD-3 could be confirmed in vitro and in vivo by co-immunoprecipitations from Sf9 insect cells. Mapping showed that CGI-55 interacts with CHD-3 via two regions at its N- and C-terminals. The CGI-55 and Ki-1/57 mRNAs show highest expression in muscle, colon and kidney. A CGI55-GFP fusion protein was localized in the cytoplasm, nucleus and perinuclear regions of HeLa cells. These data suggest the possibility that CGI-55 and Ki-1/57 might be involved in nuclear functions like the remodeling of chromatin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c74e80b61170fee2d1fd79d48131b820 https://doi.org/10.1016/s0014-5793(02)03737-7 Zobrazit plný text záznamu Plný text