Phytochemical identification and quantification of quercetin in Triplaris gardneriana wedd. leaves by HPLC-DAD with evaluation of antibacterial activity
| Autor: | Rafael Cunha Libório, Pedro José Rolim Neto, Mateus Matiuzzi da Costa, Larissa Araújo Rolim, Xirley Pereira Nunes, Sandra Kelle Souza Macêdo, Kátia Simoni Bezerra Lima, Tamires dos Santos Almeida, José Marcos Teixeira de Alencar Filho |
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| Rok vydání: | 2019 |
| Předmět: |
Chromatography
Chloroform 010405 organic chemistry Organic Chemistry Ethyl acetate Plant Science Secondary metabolite 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Column chromatography chemistry Phytochemical medicine heterocyclic compounds Antibacterial activity Quercetin Lupeol medicine.drug |
| Zdroj: | Natural Product Research. 35:3083-3088 |
| ISSN: | 1478-6427 1478-6419 |
| DOI: | 10.1080/14786419.2019.1682573 |
| Popis: | Triplaris gardneriana is used by traditional medicine. The objective of this work was the leaves chemical study with isolate, identify and quantify the chemical constituent, validate the analytical method and evaluate the antibacterial activity. The ethyl acetate and chloroform fractions were subjected to column chromatography for isolation of the compounds quercetin and lupeol, respectively. For the identification, quantification of quercetin in the samples and validation of method were performed using HPLC-DAD. The antibacterial activity was evaluated by the microdilution method. The isolated phytochemicals are being reported for the first time in the species. The ethyl acetate fraction showed a higher content of quercetin with 9.967 ± 1.01 mg.g-1. The method was validated. The samples showed good antibacterial activity. In this study, quercetin was isolated and quantified in the species being a great alternative as a producer of this secondary metabolite, which can be safely applied in the quality control analysis. |
| Databáze: | OpenAIRE |
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