Biofilm thickness variability investigated with a laser triangulation sensor
| Autor: | Spp Simon Ottengraf, B Baukje Osinga-Kuipers, Wjh Okkerse |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Laser triangulation
Chemistry Nondestructive measurement Trickling filter Biofilm Analytical chemistry Bioengineering biochemical phenomena metabolism and nutrition Bare surface Laser Applied Microbiology and Biotechnology law.invention Waste gas law Surface roughness Composite material Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 70:619-629 |
| ISSN: | 1097-0290 0006-3592 |
| DOI: | 10.1002/1097-0290(20001220)70:6<619::aid-bit3>3.0.co;2-4 |
| Popis: | Measurement of the surface roughness and thickness of biological films is laborious and usually destructive, thus hampering research in this area. We developed a laser triangulation sensor (LTS) set-up for the fast and nondestructive measurement of these biofilm parameters during growth. Using LTS measurements, the morphological development of a dichloromethane-(DCM) degrading biofilm cultured on a wetted-wall column was studied. The measurements show that the biofilm develops faster at the entrance of the reactor. The biofilm consisted of a base film in which microbial colonies were embedded. The biofilm-surface area gradually increased by 23% compaerd to the bare surface due to the formation of a large number of these colonies. The number and shape of these colonies were followed in time. Using LTS measurements, biofilms distinctly different in surface roughness could be distinguished in a laboratory trickling filter removing DCM from a waste gas. The consequences of the observed surface characteristics for the reaction-diffusion process in the biofilm and for the falling film hydrodynamics are discussed. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 619–629, 2000. |
| Databáze: | OpenAIRE |
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