Molecular genetic strain improvement for the overproduction of fungal proteins by Filamentous fungi

Autor:	C.A.M.J.J. van den Hondel, J.C. Verdoes, Peter J. Punt
Rok vydání:	1995
Předmět:	Genetics Fungal protein biology Aspergillus niger Genetic strain General Medicine Fungi imperfecti biology.organism_classification Applied Microbiology and Biotechnology Genetic recombination Transcription (biology) Overproduction Gene Biotechnology
Zdroj:	Applied Microbiology and Biotechnology. 43:195-205
ISSN:	1432-0614 0175-7598
Popis:	During the last 5 years, strain improvement by genetic engineering has been established as a good alternative for traditional methods of strain improvement such as mutagenesis and genetic recombination. Considerable success has been achieved in the overproduction of a variety of fungal proteins. However, from the available data a limitation at the level of transcription is evident. A more detailed analysis of this transcription limitation was carried out for the overproduction of glucoamylase in A. niger. This analysis revealed that both the site of integration of introduced gene copies and the available amount of trans-acting regulatory proteins were at the root of the observed limitation. Based on these results, approaches for the construction of a new generation of protein-overproducing strains are suggested.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c73eee2c0bf0cdbabc5cdd59fec330d7 https://doi.org/10.1007/bf00172812 Zobrazit plný text záznamu Full text from SpringerLink