Synthesis of 14C-ochratoxin A
| Autor: | Guido Slegers, C. Van Peteghem, D. M. Rousseau, A. Claeys |
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| Rok vydání: | 1984 |
| Předmět: |
Ochratoxin A
chemistry.chemical_classification Chromatography Chemistry Organic Chemistry food and beverages Alkaline hydrolysis (body disposal) Biochemistry High-performance liquid chromatography Hydrolysate Analytical Chemistry chemistry.chemical_compound Stereospecificity Yield (chemistry) Drug Discovery Organic chemistry Radiology Nuclear Medicine and imaging Specific activity Spectroscopy Lactone |
| Zdroj: | Journal of Labelled Compounds and Radiopharmaceuticals. 21:429-440 |
| ISSN: | 0362-4803 |
| DOI: | 10.1002/jlcr.2580210506 |
| Popis: | 14C-Ochratoxin A is obtained by coupling the N-hydroxy-succinimide derivative of the lactone acid hydrolysate of natural ochratoxin A with 14C-L-β-phenylalanine methylester. 14C-Ochratoxin A methylester is converted into 14C-ochratoxin A by alkaline hydrolysis. Purification of the intermediates and the 14C-ochratoxin A is achieved by HPLC. For an amount of 0.05 μmole L-β-phenylalanine methylester the yield of ochratoxin A is 0.02 μmole or 40% and the specific activity 130 Ci/mole. Stereospecificity is 100% and radiochemical purity is 96%. The yield of 14C-ochratoxin A increases with increasing amounts of 14C-L-β-phenylalanine methylester and reaches 80% under the best conditions. |
| Databáze: | OpenAIRE |
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