MOLECULAR DETECTION OF PROTEIN ENCODING GENES Virb11 ON BRUCELLA ABORTUS LOCAL ISOLATES ORIGIN OF PINRANG, NTT AND VACCINE STRAINS

Autor:	Suwarno Suwarno, Maria Gladis Bupu Maze, Didik Handijatno, Rahaju Ernawati, Wiwik Tyasningsih, Agnes Theresia Soelih Estoepangestie
Rok vydání:	2020
Předmět:	Brucella abortus Vaccine strain Biology bacterial infections and mycoses complex mixtures Gene Virology
Zdroj:	Jurnal Veteriner. 21:503-511
ISSN:	2477-5665 1411-8327
Popis:	Brucellosis in cattle is a disease caused by Brucella abortus due to the reduction in livestock population caused by abortion, stillbirth, weak birth, infertility and sterility. Brucella abortus has several potential virulence factors, i.e. virB11 gene that encodes VirB11 protein is an important virulence factor acts as an ATPase for assembling organelles when the bacteria replicate, helping to complete the bacterial cycle and agress to another cells. The aim of this study are to re-identification Brucella abortus and detect virB11 gene as encoding of B. abortus VirB11 protein in local isolates from Pinrang, NTT, strain vaccines S19 and RB51. The isolates Brucella abortus were re-cultured in Brucella agar base and re-identification is followed by microscopic with Gram staining and biochemical tested with urease, citrat, indol and TSIA test. virB11gene was detected with PCR method. The PCR result showed virB11 gene have DNA band 720 bp. virB11 gene are present in local isolates from Pinrang, NTT, strain vaccines S19 and RB51.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c735115e1c1555c05258f02b9ca36e52 https://doi.org/10.19087/jveteriner.2020.21.4.503 Zobrazit plný text záznamu