Magnetic Affinity Immunoassay Based Enzyme-labeled Phage Displayed Antibody

Autor:	Jin-Ping Zhang, Bing Liu, Zhi-Wei Liu, Lan-Qun Hao, Qi-Bin Huang, Xi-Hui Mu, Zhao-Yang Tong
Rok vydání:	2014
Předmět:	chemistry.chemical_classification Chromatography medicine.diagnostic_test biology Specific detection Molecular biology Analytical Chemistry Absorbance Linear relationship Enzyme chemistry Polyclonal antibodies Immunoassay medicine biology.protein Microparticle Antibody
Zdroj:	Chinese Journal of Analytical Chemistry. 42:785-790
ISSN:	1872-2040
DOI:	10.1016/s1872-2040(13)60736-7
Popis:	A novel magnetic affinity immunoassay (MAIA) strategy on the basis of enzyme-labeled phage displayed antibody was developed. The assay consisted of a sandwich format in which immobilized polyclonal antibody (pcAb) on magnetic microparticle was used as capture probe, and enzyme-labeled phage displayed antibody as specific detection probe to increase enzyme amount and enhance detection signal. By the proposed method, β-bungarotoxin (β-BGT) was successfully detected. A linear relationship between absorbance value and the concentration of β-BGT in the range of 0.016–62.5 μg L−1 was obtained. The linear regression equation was Y = 0.641X + 1.355 (R = 0.9925, n = 13, p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c734bc1731d62e3cc02371b9522697f9 https://doi.org/10.1016/s1872-2040(13)60736-7 Zobrazit plný text záznamu Full Text from ScienceDirect