FoxP3, GATA-3 and T-bet expression in elderly asthma
| Autor: | Ana Todo-Bom, Cesmi Akdis, Luísa Geraldes, Carsten B. Schmidt-Weber, Sofia Vale-Pereira, Anabela Mota-Pinto |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Senescence
medicine.medical_specialty business.industry Regulatory T cell Immunology FOXP3 chemical and pharmacologic phenomena hemic and immune systems medicine.disease Peripheral blood mononuclear cell Gene expression profiling Endocrinology Real-time polymerase chain reaction medicine.anatomical_structure Internal medicine Gene expression medicine Immunology and Allergy business Asthma |
| Zdroj: | Clinical & Experimental Allergy. 41:490-496 |
| ISSN: | 0954-7894 |
| DOI: | 10.1111/j.1365-2222.2010.03640.x |
| Popis: | Summary Background Asthma is a chronic inflammatory disorder in which Th2, Th1 and suppressive T cells (Tregs) play a role. The transcription factor FoxP3 plays a role in Treg differentiation while T-bet is important for Th1 and GATA-3 for Th2 differentiation from naive Tcells. Recent data show that age-related deregulation of Treg cells is a mechanism of senescence affecting several chronic diseases. It is crucial to understand the behaviour of these cell populations in asthma for elderly patients. Objective To evaluate FoxP3, GATA-3 and T-bet gene expression under basal conditions and after in vitro activation in a group of elderly asthmatic compared with age-matched healthy individuals. Methods Thirty-two elderly asthmatics and 17 healthy elderly individuals were selected. Serum total IgE was measured, and peripheral blood mononuclear cells (PBMCs) were isolated and stimulated in vitro with anti-CD3/anti-CD28, followed by mRNA isolation. After reverse transcription, real-time quantitative PCR was performed and relative quantification was determined 2 DDCt(2 DDCt method). Results The mean values and standard deviation of FoxP3, GATA-3 and T-bet relative |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text