Complex formation and crystallization of adrenodoxin-reeuctase with adrenodoxin

Autor:	O. Ristau, Joachim Behlke, A. Marg
Rok vydání:	2007
Předmět:	chemistry.chemical_compound chemistry law Ionic strength Sedimentation equilibrium Adrenodoxin Inorganic chemistry Crystallization Phosphate Dissociation (chemistry) Adrenodoxin reductase law.invention Carbodiimide
Zdroj:	Analytical Ultracentrifugation ISBN: 9783798510388
DOI:	10.1007/bfb0114071
Popis:	The interaction of bovine adrenodoxin reductase with adrenodoxin was investigated by means of sedimentation equilibrium technique using the analytical ultrancentrifuge XL-A. From the radial concentration distributions recorded at sedimentation equilibrium the partial concentrations of the free proteins and the complex were obtained using the program Polymol. Independent of the ratios of proteins used in the experiments a 1:1 complex was formed. The association constant amount to about 2·106 M−1 at low phosphate concentration (10–50 mM). The value drops to about 103 M−1 at 500 mM phosphate. For successfu. crystallization the complex was stabilized by cross-linking experiments with carbodiimide to prevent dissociation at higher ionic strength.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c72d113c89efb7ffbb38adb9abcbba49 https://doi.org/10.1007/bfb0114071 Zobrazit plný text záznamu