Production of four commercially cultivatedEchinaceaspecies by different methods ofin vitroregeneration
| Autor: | Prakash Lakshmanan, Majid Danesh, Acram Taji |
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| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | The Journal of Horticultural Science and Biotechnology. 77:158-163 |
| ISSN: | 2380-4084 1462-0316 |
| Popis: | SummaryEfficient in vitro procedures for mass propagation of four commercially important Echinacea species have been deveoped. Plants of E. angustifolia, E. pallida, E. paradoxa and E. purpurea were regenerated by three methods, namely axillary bud proliferation, adventitious shoot formation and somatic embyrogenesis. Shoot tips obtained from in vitro germinated seedlings, adventitious shoots or somatic embryo-derived plantlets, when cultured on Murashige and Skoog medium enriched with 1 μM 6-benzylaminopurine, 2 μM kinetin, 0.5 μM indole-3-butyric acid and 4 mg–1 paclobutrazol multiplied three-fold within 3–4 weeks in culture. Incorporation of paclobutrazol in the shoot multiplication medium was necessary to recover healthy and robust shoots suitable for rooting. Direct, high-frequency shoot formation on intact leaves of shoots grown on 6-benzylaminopurine and kinetin-supplemented media, an unusual and novel observation made in this study, occurred in all the species studied. Rooting of in vitro develope... |
| Databáze: | OpenAIRE |
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