Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.)

Autor:	R. A. Schilperoort, M. Leneman, S. Rueb, L. A. M. Hensgens
Rok vydání:	1994
Předmět:	Oryza sativa Somatic embryogenesis fungi food and beverages Plant physiology Horticulture Biology Scutellum musculoskeletal system biology.organism_classification Japonica body regions surgical procedures operative Plant morphology Callus Botany Cultivar
Zdroj:	Plant Cell, Tissue and Organ Culture. 36:259-264
ISSN:	1573-5044 0167-6857
Popis:	To obtain a reproducible efficient procedure for regeneration of rice plants through somatic embryogenesis from callus four published methods of callus induction and regeneration were compared. Callus was initiated from mature embryos of the Japonica cultivar Taipei 309 of rice (Oryza sativa L.). The number, mass and morphology of the callus formed on the scutellum were dependent on the medium used. A limited humidity and an optimal aeration of the culture vessels enhanced the frequency of embryogenesis and plant regeneration. A method described by Poonsapaya et al. (1989) was found to be the most efficient and was slightly modified. As a result 98% of the T309 embryos formed callus, of which 63% regenerated into plants. Each callus yielded an average of 6 plants. Plant morphology, fertility and seed set of the regenerants were found to be normal.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c7188c9abbff6f3015c1bb53e7b99312 https://doi.org/10.1007/bf00037729 Zobrazit plný text záznamu Full text from SpringerLink