Extinction of the human insulin gene expression in insulinoma X fibroblast somatic cell hybrids involves cis-acting DNA elements

Autor:	Jacques Jami, Dominique Daegelen, Eliane Monthioux, Corinne Besnard, Patrick Loràs
Rok vydání:	1991
Předmět:	Physiology Insulin medicine.medical_treatment Clinical Biochemistry Rat Insulinoma Cell Biology Biology Molecular biology chemistry.chemical_compound medicine.anatomical_structure chemistry Regulatory sequence Transcription (biology) Gene expression medicine Fibroblast Gene DNA
Zdroj:	Journal of Cellular Physiology. 146:349-355
ISSN:	0021-9541
Popis:	Insulin gene expression in rat insulinoma (RIN) cells is extinct in RIN × fibroblast hybrids and can reappear upon loss of DNA contributed by the fibroblast parent. (Besnard et al., Exp. Cell Res. 185:101–108, 1989). In the present study, we looked for the role of 5′-flanking sequences of the human insulin gene in the negative control observed in the hybrids. RIN cells were transformed with composite genes which consisted of the coding sequence of the gpt gene placed under the control of 5′-flanking regions of the human insulin gene (Ins. gpt gene). Upon hybridization of these cells with mouse fibroblasts, the expression of both Ins. gpt and endogenous rat insulin genes were suppressed together. The results obtained indicate that cis-acting DNA elements are involved in the negative control of the gene. These elements are located in a fragment spread from -258 to +241 of the transcription origin of the human insulin gene.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c71062a9d091fe041b921371e204132c https://doi.org/10.1002/jcp.1041460303 Zobrazit plný text záznamu Plný text