| Autor: |
Bonnie Dillon, Alan S. Unis, Jennifer Vincent |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Progress in Neuro-Psychopharmacology and Biological Psychiatry. 19:333-342 |
| ISSN: |
0278-5846 |
| DOI: |
10.1016/0278-5846(95)00014-m |
| Popis: |
1. 1. Striatal D 2 receptor concentration (as determined by [ 3 H]-spiperone binding to brain tissue homogenates) has been correlated, in F344 rats, with a number of locomotor behaviors (e.g. response latency on conditioned avoidance and decreased open field locomotion with apomorphine administration). 2. 2. These data suggest that striatal D 2 receptor concentration may likewise correlate with spontaneous open field locomotor activity since previous work has shown lower rates of locomotor activity in 21-day old versus 30-day old F344 rats. 3. 3. Using the technique of in vitro receptor autoradiography to slide-mounted, coronal brain sections, the authors studied the suitability of [ 3 H]-YM-09151-2 as a ligand for labeling D2 receptors in 21-day old and adult F344 rats. 4. 4. Specific [ 3 H]-YM-09151-2 saturation binding accounted for 70–80% of the total bound ligand ( K d = 348 pM). Displacement experiments corroborated previous findings using homogenates that [ 3 H]-YM 09151-2 binds specifically to the D 2 receptor. 5. 5. Autoradiograms from 21-day old and adult brain sections demonstrated high grain densities in the caudate-putamen, accumbens and olfactory tubercle. Diffuse specific binding was also observed in the cortex. Analysis of grain densities from areas corresponding to these brain regions revealed significantly less specific binding in 21-day old versus adult F344 rats. 6. 6. These autoradiographic data are consistent with the expected increase in brain D 2 receptor concentration during maturation and parallel observed changes in open-field locomotor activity. Thus, [ 3 H]-YM 09151-2 appears to be a suitable ligand for autoradiographic labeling of the D 2 receptor. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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