Optimized CUBIC protocol for 3D imaging of chicken embryos at single-cell resolution
| Autor: | Manuel Desco, Maria Victoria Gómez-Gaviro, Juan José Vaquero, Diana Bocancea, Jorge Ripoll, Evan Balaban, Maria Pompeiano, María Teresa Lorrio |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
animal structures Tissue clearing Resolution (electron density) Embryo Anatomy Biology 03 medical and health sciences 030104 developmental biology Three dimensional imaging Single-cell analysis Light sheet fluorescence microscopy embryonic structures Microscopy Molecular Biology Developmental Biology Biomedical engineering |
| Zdroj: | Development. |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.145805 |
| Popis: | The CUBIC tissue clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell-level resolution in whole early-stage chicken embryos, and in the whole brains of late-stage embryos. |
| Databáze: | OpenAIRE |
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